CRISPR achievements

[Avalancheon]

Hi guys, its been a while since I've posted here. Had a busy year

I wanted to ask a question about the CRISPR/CAS method of gene editing.

Do any of you know whether there a vector system compatible with CRISPR, such that it can be used for in vivo genetic modification? If not, how long will it be before such a development takes place?

I think this is a major technical barrier that must be surmounted before we can have a viable method of gene editing.

[Rynn]

In vivo CRISPR gene therapy is already being done in the lab. Check out section 4 of this review for an outline of methods, their progress and limitations:

https://www.ncbi.nlm.nih.gov/pmc/article...po=34.4340

[Avalancheon]

In vivo CRISPR gene therapy is already being done in the lab. Check out section 4 of this review for an outline of methods, their progress and limitations:

https://www.ncbi.nlm.nih.gov/pmc/article...po=34.4340

Interesting paper. So there is some progress being made on the delivery strategys of CRISPR. All of the currently used vectors seem to have limitations, though. The adeno-associated virus (AAV) was stated to have a 'small cargo size'.

Out of all the possible vectors named in that study, which do you think is the most relevant in the long term? Would you put your money on the adenoviral and lentiviral vectors, or on the lipid and polymer based vectors?